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原花青素B2对过氧化氢诱导人脐静脉内皮细胞株EA.hy926氧化损伤的影响研究

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  • R543

摘要:

目的 探究原花青素B2(PB2)对过氧化氢(H2O2)诱导的人脐静脉内皮细胞株EA.hy926
氧化损伤的影响及其可能机制。方法 体外培养人脐静脉内皮细胞株EA.hy926,将细胞随机分为5组,
即对照组、H2O2组、α-生育酚组(30 μM α-tocopherol)、2 μM PB2组及10 μM PB2组,分别给予
不同处理。对照组仅加入细胞培养液处理;H2O2组在对照组基础上加用H2O2,终浓度为200 μmol/L;
α-生育酚处理组在对照组基础上加用H2O2及α-生育酚(α-tocopherol),终浓度分别为200 μmol/L
及30 μmol/L;2 μM PB2组在对照组基础上加用H2O2及PB2,终浓度分别为200 μmol/L 及2 μmol/L;
10 μM PB2组在对照组基础上加用H2O2及PB2,终浓度分别为200 μmol/L及10 μmol/L。对各组进行人
类EA.hy926内皮细胞的生长分析及PB2对H2O2诱发人类EA.hy926内皮细胞DNA损伤、脂质过氧化和活性
氧(ROS)的影响检测。结果 与对照组比较,H2O2处理组的细胞数目明显降低,差异有统计学意义(P
<0.05);与H2O2组相比,α-生育酚处理组、2 μmol/L和10 μmol/L PB2处理组的细胞数目均明显增
加,差异均有统计学意义(P均<0.05)。与对照组比较,H2O2处理组细胞DNA损伤显著,差异有统计学
意义(P<0.05);与H2O2处理组对比,2 μmol/L PB2处理组、10 μmol/L PB2处理组、α-生育酚处理组
对H2O2所诱发的DNA损伤明显抑制,差异有统计学意义(P<0.05),抑制率分别为24%、55%和95%。
与对照组比较,H2O2处理组的丙二醛(MDA)、ROS水平显著上升,差异有统计学意义(P<0.05)。与
H2O2处理组对比,2 μmol/L PB2处理组、10 μmol/L PB2处理组和α-生育酚处理组明显抑制H2O2诱发人
类EA.hy926内皮细胞MDA、ROS的产生,差异均有统计学意义(P<0.05),抑制率分别为21%、42%、
49%和18%、41%、80%。结论 PB2能够显著抑制H2O2诱发的人类EA.hy926内皮细胞ROS的生成,降低氧
化损伤从而达到保护内皮细胞的效果。

Abstract:

Objective To study the influence of procyanidine B2 (PB2) on oxidative damage of human
umbilical vein endothelial cell line (EA.hy926) induced by hydrogen peroxide (H2O2) and possible mechanism.
Methods EA.hy926 were cultured in vitro, and divided randomLy into 5 groups: control group, H2O2 group,
α-tocopherol group (30 μM), PB2 group 1 (2 μM) and PB2 group 2 (10 μM). The control group was treated with
DMEM, H2O2 group was treated with DMEM and H2O2 (final concentration=200 μmol/L), α-tocopherol group was
treated with DMEM, H2O2 (final concentration=200 μmol/L) and α-tocopherol ((final concentration=30 μmol/L),
PB2 group 1 was treated with DMEM, H2O2 (final concentration=200 μmol/L) and PB2 (final concentration=2
μmol/L), and PB2 group 2 was treated with DMEM, H2O2 (final concentration=200 μmol/L) and PB2 (final
concentration=10 μmol/L). The growth of EA.hy926 was analyzed, and influence of PB2 on DNA injury, lipid
peroxiation and reactive oxygen species (ROS) of EA.hy926 induced by H2O2 was detected. Results Compared with
control group, the number of EA.hy926 decreased significantly in H2O2 group (P<0.05). Compared with H2O2 group,
the number of EA.hy926 increased significantly in α-tocopherol group, PB2 group 1 and PB2 group 2 (all P<0.05).
Compared with control group, DNA injury of EA.hy926 was more significant in H2O2 group (P<0.05). Compared
with H2O2 group, DNA injury of EA.hy926 was significantly inhibited in PB2 group 1, PB2 group 2 and α-tocopherol
group (P<0.05), and inhibition rate was, respectively, 24%, 55% and 95%. Compared with control group, the levels

of malondialdehyde (MDA) and ROS increased significantly in H2O2 group (P<0.05). Compared with H2O2 group,

the productions of MDA and ROS in EA.hy926 induced by H2O2 were significantly inhibited in PB2 group 1, PB2
group 2 and α-tocopherol group (P<0.05), and the inhibition rate was, respectively, 21%, 42% and 49%, and 18%,
41% and 80%. Conclusion PB2 can significantly inhibit the production of ROS in EA.hy926 induced by H2O2,
relieve oxidative damage and protect endothelial cells.

基金项目:

中国医学科学院医学与健康科技创新工程(2016-12M-1-011)

参考文献:

[1] 马丽媛,吴亚哲,王文,等. 《中国心血管病报告2017》要点解读[J].
中国心血管杂志,2018,23(1):3-6.
[2] Dupré-Crochet S,Erard M,Nüβe O. ROS production in phagocytes:
why, when, and where?[J]. J Leukoc Biol,2013,94(4):657-70.
[3] Hort MA,Straliotto MR,Duz MS,et al. Cardioprotective effects of a
proanthocyanidin-rich fraction from Croton celtidifolius Baill: focus
on atherosclerosis[J]. Food Chem Toxicol,2012,50(10):3769-75.
[4] Cádiz-Gurrea ML,Borrás-Linares I,Lozano-Sánchez J,et al. Cocoa
and Grape Seed Byproducts as a Source of Antioxidant and Anti-
Inflammatory Proanthocyanidins[J]. Int J Mol Sci,2017,18(2). pii: E376.
[5] 常成成,位庚,李红蓉,等. 微血管内皮细胞损伤在心血管疾病发病中
的作用及通络干预研究进展[J]. 时珍国医国药,2015,26(6): 1459-62.
[6] 赵海梅,杨斌,成蓓. 衰老血管内皮细胞线粒体膜电位与活性氧的
变化[J]. 中国组织工程研究,2013,17(15):2729-34.
[7] 张文晓,尚静,郜鑫,等. 1,4-萘醌老化黑碳对人支气管上皮细胞活性
氧和DNA链断裂的影响[J]. 北京大学学报:医学版,2015,47(4): 690-6.
[8] Spirlandeli AL,Deminice R,Jordao AA. Plasma malondialdehyde as
biomarker of lipid peroxidation: effects of acute exercise[J]. Int J
Sports Med,2014,35(1):14-8.
[9] 林艾雯,陈竹君. 动脉粥样硬化与内皮细胞损伤机制的研究进展
[J]. 岭南心血管病杂志,2015,21(4):580-2.
[10] Shibata A,Nakagawa K,Tsuduki T,et al. α-Tocopherol suppresses
antiangiogenic effect of δ-tocotrienol in human umbilical vein
endothelial cells[J]. J Nutr Biochem,2015,26(4):345-50.
[11] 张怀民,杨虹,郑海洲. 天然黄酮类化合物防治心脑血管疾病的研
究进展[J]. 中国新药与临床杂志,2016,35(10):704-8.
[12] Leyva-Soto A,Chavez-Santoscoy RA,Lara-Jacobo LR,et al. Daily
Consumption of Chocolate Rich in Flavonoids Decreases Cellular
Genotoxicity and Improves Biochemical Parameters of Lipid and

Glucose Metabolism[J]. Molecules,2018,23(9). pii:E2220.

[13] Ioannou Y,Zhang JY,Passam FH,et al. Naturally occurring free thiols
within beta 2-glycoprotein I in vivo: nitrosylation, redox modification
by endothelial cells, and regulation of oxidative stress-induced cell
injury[J]. Blood,2010,116(11):1961-70.
[14] Wang B,Zhang Q,Yao R,et al. 7, 8-Dihydroxyflavone Protects an
Endothelial Cell Line from H2O2 Damage[J]. PLoS One,2015,10(8):
e0135345.
[15] Chen Q,Wang Q,Zhu J,et al. Reactive oxygen species: key regulators
in vascular health and diseases[J]. Br J Pharmacol,2018,175(8):
1279-92.
[16] 康贵龙,杨才弟. 荨麻疹患者外周血丙二醛、单一核细胞DNA损
伤及总抗氧化能力水平与病情相关性研究[J]. 临床皮肤科杂志,
2015,44(5):276-8.
[17] Raudone L,Raudonis R,Liaudanskas M,et al. Phenolic Profiles and
Contribution of Individual Compounds to Antioxidant Activity of
Apple Powders[J]. J Food Sci,2016,81(5):C1055-61.
[18] Yang H,Xiao L,Yuan Y,et al. Procyanidin B2 inhibits NLRP3
inflammasome activation in human vascular endothelial cells[J].
Biochem Pharmacol,2014,92(4):599-606.

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